Fig 2: Bioinformatics analysis. (A, B) Expression pattern of SETD7 in CRC based on datasets in TCGA and GEO. (C, D) Expression pattern of FGA in CRC based on datasets in TCGA and GEO. (E) Expression pattern of MUC5AC in CRC based on datasets in GEO. (F) Expression pattern of SETD7 in gastric cancer based on datasets in TCGA. (G) Expression pattern of SETD7 in hepatic cancer based on datasets in TCGA. (H) Expression pattern of SETD7 in lung cancer based on datasets in TCGA. (I) Expression pattern of SETD7 in pancreatic cancer based on datasets in GEO. (J) Expression pattern of SETD7 in breast cancer based on datasets in GEO.

Fig 3: ELISA and IHC analysis of SETD7 in serum and tissues, respectively. (A) SETD7 expression in the serum of different groups. (B-D) SETD7 expression in para-carcinoma, colorectal polyps and CRC tissue, respectively. (E) Positive expression area of SETD7 in para-carcinoma, colorectal polyps and CRC tissue. (*** indicates P < .001, ** indicates P < .01, * indicates P < .05). (F, G) SETD7 expression in T1 and T3 stage, respectively. (H, I) SETD7 expression in MSS/MSI-L and MSI-H, respectively (figures are magnified ×200, and the upper right corner is magnified ×400).

Fig 4: Bioinformatics analysis. (A) Expression pattern of SETD7 in esophageal cancer based on datasets in TCGA. (B) Expression pattern of SETD7 in pancreatic cancer based on datasets in TCGA. (C) Expression pattern of SETD7 in breast cancer in CRC based on datasets in TCGA. (D) Expression pattern of SETD7 in esophageal cancer based on datasets in GEO. (E) Expression pattern of SETD7 in gastric cancer based on datasets in GEO. (F) Expression pattern of SETD7 in hepatic cancer based on datasets in GEO.(G) Expression pattern of SETD7 in lung cancer based on datasets in GEO.

Fig 5: The effect of SETD7 knockdown (A, B) Cell viability of SETD7 knockdown HCT116 and RKO cells. (C, D) Cell cycle analysis of SETD7 knockdown HCT116 and RKO cells. (E-L) Cell apoptosis analysis of SETD7 knockdown HCT116 and RKO cells. (*** indicates P < .001, ** indicates P < .01, * indicates P < .05).